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. 2021 May 13;11:10270. doi: 10.1038/s41598-021-89464-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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KEGG enrichment analysis for DE genes. (A–D) Top 20 KEGG pathways enriched by DE genes in B vs A, C vs A, D vs A, and E vs A groups, respectively. Pathway in the Y-axis represents the KEGG pathway term enriched by differentially expressed genes. The “rich” in the X-axis represents the rich factor. Rich factor: the number of differentially expressed genes in the pathway term/the number of all annotated genes in the pathway term. FDR: P value after the correction of False Discovery Rate (FDR). DEG_number: number of differentially expressed genes in the KEGG pathway term. KEGG enrichment analysis was performed as previously described^48–50.