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. 2021 May 13;11:10270. doi: 10.1038/s41598-021-89464-1

Figure 6.

Figure 6

MiR-6631-5p overexpression promoted LaSota NDV replication in DF-1 cells. (A) The level of gga-miR-6631-5p was measured by RT-qPCR assay in DF-1 cells infected with or without LaSota NDV at the indicated time points after infection. (B) DF-1 cells were transfected with gga-miR-6631-5p mimic (100 nM) or NC mimic (100 nM). At 48 h after transfection, gga-miR-6631-5p level was determined by RT-qPCR assay. (C) DF-1 cells were transfected with gga-miR-6631-5p mimic (100 nM) or NC mimic (100 nM). Non-transfected cells acted as the control group. At 24 h after transfection, cells were infected with LaSota NDV at the MOI of 1. At the indicated time points (12, 24, 36, 48 h) after LaSota infection, TCID50 assay was performed to examine the effect of gga-miR-6631-5p overexpression on LaSota NDV replication.