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. 2021 May 13;12:2768. doi: 10.1038/s41467-021-22980-w

Fig. 2. ProIL2 effectively masks IL-2 activity and is cleaved by MMPs in vitro.

Fig. 2

a Schematic of ProIL2 depicting that IL-2 receptor beta (IL2RB) binds and blocks IL-2 activity prior to cleavage (left) and activates into SumIL2-Fc upon MMP cleavage (right). b Binding of SumIL2-Fc and ProIL2 at different doses to HEK-BlueTM IL-2 reporter cells was assessed via flow cytometry (n = 3 experiments, total 3 replicates per group). c Functional activity of SumIL2-Fc and ProIL2 was assessed by using HEK-BlueTM IL-2 reporter cell assay (n = 3 experiments, total 9 replicates per group). d Protease activation of ProIL2. ProIL2 was incubated with human MMP2, MMP9, and MMP14 for 1 or 4 h, then run on non-reducing SDS-PAGE, where gel band intensity was subsequently quantified (n = 3 experiments). e Functional activity of SumIL2-Fc, ProIL2, and ProIL2 that was incubated with human MMP2, MMP9, and MMP14 for 4 h was assessed by using HEK-BlueTM IL-2 reporter cell assay (n = 1 representative experiment of 2 experiments, 3 replicates each group per experiment, total 6 replicates). Data represent mean ± s.e.m. Source data are provided as a Source Data file.