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. 2021 Apr 30;15:671473. doi: 10.3389/fncel.2021.671473

Figure 3.

Figure 3

Dynamics of extracellular dopamine in the nucleus accumbens (NAc) in response to sevoflurane anesthesia. (A) Schematic diagram depicting the fiber photometry recording of extracellular dopamine. (B) Expression of DA2 h in NAc from each cases are outlined. Timeline for dynamics of extracellular dopamine recording in sevoflurane anesthesia. (C) Expression of the DA2h virus in the NAc area (Scale bars: 200 μm). (D) Heat map illustrating the changes in the DA fluorescence signals related to sevoflurane-induced LORR and RORR. (E) Average DA transmitters transients associated with LORR and RORR; mean (red trace) ± SEM (red shading). Note that the extracellular DA fluorescence signals gradually decreased with LORR and increased sharply after RORR. (F) ΔF/F represents the deviation in the DA transmitter signal from the baseline, which is the averaged ΔF/F between t = –300 s and –150 s. Data are presented as the mean ± SEM. **p < 0.01; ***p < 0.001; ****p < 0.0001, n = 10, by paired t-test.