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. 2021 Apr 30;8:645378. doi: 10.3389/fcvm.2021.645378

Figure 5.

Figure 5

CircHIPK3 sponges miR-93-5p to activate the Rac1/PI3K/AKT pathway. (A) The website predicted the targeting relationship between miR-93-5p and Rac1. (B) Dual-luciferase assay was used to verify the targeting relationship between miR-93-5p and Rac1. (C) RT-qPCR was used to detect the expression of Rac1 in MI model in vitro and in vivo. (D) RT-qPCR was used to detect the levels of CircHIPK3, miR-93-5p, and Rac1. (E) Western blot analysis was used to detect the protein levels of p-PI3K and p-AKT. The value in the figures is the measurement data, which is expressed as mean ± standard deviation. The comparison between two groups was analyzed by independent-sample t-test. The comparison among multiple groups was analyzed by one-way ANOVA, followed by Tukey's multiple-comparisons test. *p < 0.05; #p < 0.05; &p < 0.05. The experiment was repeated three times.