FIGURE 4.

MFQ shows synergistic anti-SARS-CoV2 activity with replication inhibitor NFV. (A) Viral RNAs in the culture supernatant at 24 h after co-treatment with MFQ and NFV were quantified by real-time RT-PCR. Relative values are shown of viral RNA or cell viability to those treated with DMSO control. Cell viability was simultaneously measured with a high content image analyzer. [MFQ at 0, 0.83, 1.08, 1.40, 1.82, and 2.37 μM (1.3-fold-dilution); NFV at 0, 2.20, 2.64, and 3.17 μM (1.2-fold-dilution)]. (B) The three-dimensional interaction landscapes of NFV and MFQ were evaluated with the Bliss independence model. Orange, white and dark-blue colors on the contour plot indicate synergy, additive and antagonism, respectively.