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. 2021 May 13;12:2776. doi: 10.1038/s41467-021-23086-z

Fig. 2. Inhibition and degradation of NETs improves radiation response.

Fig. 2

a Schematic representation of tumor growth experiment, where C57BL/6 or PAD4−/− mice were injected subcutaneously (s.c) in the right flank with MB49 (500,000 cells). Mice were randomized into two groups: non-irradiated (control) or irradiated (RT). Tumors were irradiated with two fractions of 5 Gy and DNAse I was administered intramuscularly (i.m.) to degrade NETs or NEi was administered through oral gavage to inhibit NETs. b Tumor growth kinetics 11 days post-RT (n = 8 mice per arm). Data represented as mean ± SEM, two-way ANOVA with Bonferroni’s multiple comparison’s test was used to assess statistical significance. c Kaplan–Meier percent survival, log rank (Mantel-cox) test. NS = not significant p < 0.05 (C57BL/6 vs. PAD4−/− p = 0.28, C57BL/6 + DNAse I p = 0.07, C57BL/6 NEi p = 0.10, PAD4−/− RT vs. C57BL/6 RT + DNAse I p = 0.34, PAD4−/− RT vs. C57BL/6 RT + NEi p = 0.06, C57BL/6 RT + DNAse I vs. C57BL/6 RT + NEi p > 0.99), ***p < 0.001.