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. 2021 May 13;12:2776. doi: 10.1038/s41467-021-23086-z

Fig. 3. HMGB1 promotes NET formation through TLR4.

Fig. 3

a Stimulation of human PMNs (N = 5 PMNs isolated from different healthy donors) or murine PMNs (N = 3) with rHMGB1 significantly induces NETs measured by Sytox green fluorescence ***p < 0.001. b Representative fluorescence images through confocal microscopy (10×), scale bar 100 μm. c Stimulation of human PMNs (n = 5 PMNs isolated from different healthy donors) with irradiated UM-UC3 conditioned media or murine PMNs (n = 3) with irradiated MB49 conditioned media significantly induces NETs and this is reversed through addition of GLZ. d Representative fluorescence images through confocal microscopy (10×), scale bar 100 μm. e Stimulation of murine PMNs from TLR4−/− mice stimulated with rHMGB1 (n = 3) or f irradiated MB49 conditioned media (n = 3), and g representative fluorescence images through confocal microscopy (10×), scale bar 100 μm. All data are represented as mean ± SEM of triplicates from individual experiments, paired-student’s t-test was used to assess statistical significance (a, c, e, f).