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Determination of population doubling time (hours). MSCs from each of three donors (n = 3) were plated at 100,000 cells/well on 6-well plates and cultured in different serum sources for 72 h. Population doubling time was determined for each cell line individually and overall, as described in Methods, and presented graphically (A) and numerically (B). *Statistical significance assessed at p < 0.05. ns, non-significant statistical analysis.
