Fig. 1.

Dedicator of cytokinesis 2 (Dock2) regulates the expression of chemokines in macrophages after Citrobacter rodentium infection. (a–e) Reverse transcription–quantitative polymerase chain reaction (RT–qPCR) analysis of the gene expression of chemokine (C‐C motif) ligand (CCL)4, CCL5, stromal cell‐derived factor (SDF‐1β), monocyte chemoattractant protein (MCP)‐1 and CCL22 in wild‐type (WT) and Dock2^−/− bone marrow‐derived macrophages (BMDM) that were either uninfected or infected with C. rodentium for 2, 4 and 8 h. (f–j) Enzyme‐linked immunosorbent assay (ELISA) analysis of the protein levels of CCL4, CCL5, SDF‐1β, MCP‐1 and CCL22 in the supernatants from WT and Dock2^−/− BMDM that were either uninfected or infected with C. rodentium for 8 h. Data were representative of two independent experiments [mean ± standard error of the mean (s.e.m.)]. Data were analyzed by two‐way analysis of variance (anova) and two‐tailed t‐test at each time‐point. **P < 0·01; ***P < 0·001; ****P < 0·0001; n.s. = not statistically significant.