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. 2021 Mar 9;204(3):396–410. doi: 10.1111/cei.13587

Fig. 7.

Fig. 7

NLR family pyrin domain containing 3 (NLRP3) over‐expression reversed the effect of miR‐223‐3p mimic on the viability, pyroptosis and the expression of NLRP3 in monosodium urate (MSU)‐induced fibroblast‐like synoviocytes (FLSs). (a) The expression of NLRP3 in the rat FLSs after treatment with MSU and transfection with miR‐223‐3p mimic or NLRP3 was detected by reverse transcription–quantitative polymerase chain reaction (RT–qPCR); β‐actin was used as an internal control; (b) the expression of NLRP3 in the rat FLSs after treatment with MSU and transfection with miR‐223‐3p mimic or NLRP3 was detected by immunofluorescence; (c) the viability of the rat FLSs after treatment with MSU and transfection with miR‐223‐3p mimic or NLRP3 was detected by methyl thiazolyl tetrazolium (MTT); (d) the pyroptosis of the rat FLSs after treatment with MSU and transfection with miR‐223‐3p mimic or NLRP3 was detected by flow cytometry (* P < 0·05, *** P < 0·001, versus MSU; # P < 0·05, ## P < 0·01, versus MSU + M + NC). M: miR‐223‐3p mimic; NC: negative control.