FIGURE 1.

The process of experimental operation. (a) Timeline of the experiment. A total of 36 male Sprague Dawley (SD) rats aged PND 21 were used in the experiment. Rats were treated with drug from PND 22 to PND 52. After 31 days of treatment, half of the rats (18 rats) were used to performed the novel object recognition test on PND 53 and PND 54 and then were sacrificed on PND 55 for immunohistochemistry test; the other half of the rats (18 rats) were used to performed the open field test on PND 53 and then were sacrificed on PND 54 for biochemical analysis. PND, postnatal day; OFT, open field test; NOT, novel object recognition test. (b) The experimental procedure of the novel object recognition test. In the training trial, each rat was placed in the square box with two identical objects for 5 min. After 1 hr, the testing trial was conducted, and each rat was placed back into the test box for another 5 min with one of the two identical objects replaced by a novel object. Choice of the objects to be explored and their positions were decided according to a random number table