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. 2021 Apr 30;12:656846. doi: 10.3389/fimmu.2021.656846

Figure 1.

Figure 1

Visualization of FlowSOM and diffcyt results obtained through comparing CD3 T cells stimulated with donor antigen from pre and post KT. Panels on the left (A–C) contain data obtained from comparing pre- vs post-KT donor-stimulated T cells in N=9 elderly recipients while those on the right (D–F) resulted from comparing pre- vs post-KT donor-stimulated T cells in N=8 young KT recipients. (A, D) Minimal spanning trees (MST) represent the 625 clusters created with FlowSOM. Each ‘node’ representing a cluster contains a pie chart which illustrates the mean marker values. The nodes placed near each other have similar marker expression profiles. (B, E) Volcano plots of diffcyt results for each FlowSOM cluster showcasing the negative log base 10 of the adjusted p-values on the y axis and log base 2 of the fold change from pre to post KT on the x axis. TopDA clusters with a p adjusted value below 0.05 (highly significant) are highlighted in red. (E, F) The median fluorescence intensity (MFI) expression profile of each topDA cluster is illustrated in a heatmap with rows representing each cluster and columns the markers of interest. ns, non-significant.