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. 2021 Apr 30;12:656846. doi: 10.3389/fimmu.2021.656846

Figure 5.

Figure 5

CD4+CD137+ TIGIT+ T cells secrete cytokines in a polyfunctional manner. (A) PBMCs obtained from 13 responders (4 healthy individuals and 9 kidney transplant recipients pre transplantation) following stimulation with alloantigen. A representative sample illustrates how CD4+CD137+ T cells were gated then analyzed for co-expression of TIGIT and the cytokines, TNF-α, IL-2 and IFN-γ. In brackets is shown the fraction of TIGIT+/- within CD137+CD4+ T cells which express the cytokine. (B) The percentage of cytokine-producing CD4+CD137+ T cells either positive or negative for TIGIT which produce IL-2, TNF-α and/or IFN-γ. (C) The proportion of cytokine producing CD4+CD137+ T cells within the TIGIT positive or negative portions which produce one (single), two (double) or all (triple) of the cytokines, TNF-α, IL-2 and IFN-γ. (D) The combinations of TNF-α, IL-2 and IFN-γ cytokines produced by CD4+CD137+ T cells divided according to TIGIT expression.