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Kinetic parameters [fructose-1,6-bisphosphate (FBP) cleavage] of native FBAs and site-directed mutagenesis (SDMs) in glycolysis.
| Sample | Vmax (U mg−1) | KM (FBP, mM) | kcat (s−1) | Catalytic efficiency (s−1 mM−1) |
|---|---|---|---|---|
| FBAC | 4.2 | 0.3 | 2.1 | 6.9 |
| FBAC; E51V | 2.8 | 0.2 | 1.4 | 7.2 |
| FBAC; T140R | 2.4 | 0.7 | 1.3 | 1.8 |
| FBAC; E51V,T140R | 2.2 | 1.0 | 1.1 | 1.1 |
| FBAP | 1.8 | 3.4 | 1.0 | 0.3 |
| FBAP; V51E | 0.5 | 5.8 | 0.3 | 0.1 |
| FBAP; R140T | 0.4 | 4.7 | 0.2 | <0.1 |
| FBAP; V51E, R140T | 0.01 | <0.01 | <0.01 | <0.01 |
