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. 2021 May 13;12(5):483. doi: 10.1038/s41419-021-03765-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A CCK-8 assay for the proliferation of chondrocytes 1, 3, 5 and 7 days after KGN treatment. B RT-PCR analysis for the expression levels of COL2A1 and ACAN mRNA in chondrocytes after KGN treatment. C Immunofluorescence analysis for the expression of COL II proteins in chondrocytes after KGN treatment. (Scale bar = 100 μm). D RT-PCR for the expression levels of MMP13 and ADAMTS5 mRNA in chondrocytes after KGN treatment. E, F Western blot analysis for the expression of COL II, aggrecan, MMP13 and ADAMTS5 proteins in chondrocytes after KGN treatment. Values represent mean ± S.E.M of six replicas for cell proliferation assays, four replicas for RT-PCR experiments and three replicas for Western blot assays, respectively. (*p < 0.05 and **p < 0.01; between the indicated groups and ^#p < 0.05 or ^##p < 0^.01 versus the CTRL group).