Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 13;11:10200. doi: 10.1038/s41598-021-89655-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Antiproliferative effects of the two-drug combination are better than those of the single-drug combination in the RCC-24, 786-O and Caki-2 cell lines. (A) Combination effect of Ketoconazole and sunitinib on cell proliferation determined by using the MTT assay. Cells were treated with the indicated concentrations of Ketoconazole and sunitinib, and proliferation was determined 48 h later. (B) Clonogenic assay was performed in the 293-T, RCC-24, 786-O and Caki-2 cell lines. Colonies were counted 14 days later. Data are presented as the means ± SD from three independent experiments and shown in bar graphs (*p < 0.05 vs. K − K + S or S − K + S). (C) Note a significant decrease in cyclin D1 levels after sunitinib treatment and its enhancement in combination treatment with ketoconazole. The densitometric plots shows fold decrease in the expression of signaling molecule. GAPDH immunoblot used as loading control. Full blots are presented in Supplementary Fig. S6.