Fig. 2. Collecting animal behavior trajectories via a 3D motion-capture system.

a Pipeline of 3D animal skeletal reconstruction. b Center, schematic diagram of recording animal behavior with four synchronized cameras; corners, frames captured by the cameras with the DLC labels (left) and the corresponding reconstructed skeletons (right). c Left: 16 key body parts include the nose, left ear, right ear, neck, left front limb, right front limb, left hind limb, right hind limb, left front claw, right front claw, left hind claw, right hind claw, back, root tail, middle tail, and tip tail. Right: representative mouse body tracking trace data collected over 100 s showing 48 data vectors obtained by DLC for each body part (indicated with a color-coded dot) encoded by x, y, and z coordinates. For visualization purposes, mean normalization is applied to each trace. d 3D reconstruction quality assessment: 1—best quality, 0—worst quality. The quality of the data obtained from the 12 mice averaged at 0.9981 ± 0.001. Source data are provided as a Source Data file.