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. 2021 May 13;12:2780. doi: 10.1038/s41467-021-23013-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Schematic representation of the viral DNA forms during the infection. VS: viral strand; CS: complementary strand; ssDNA: single-stranded DNA; dsDNA: double-stranded DNA. b, c, d, e Accumulation of complementary strand (CS) (b, c) and viral strand (VS) (d, e) during local TYLCV infections in POLA2-silenced (TRV-NbPOLA2), POLD2-silenced (TRV-NbPOLD2), POLE2-silenced (TRV-NbPOLE2), or empty vector control (EV) N. benthamiana plants, measured by qPCR at 3 days post inoculation. Data are the mean of six independent biological replicates; error bars represent SD. The 25S ribosomal DNA interspacer (ITS) was used as a reference gene; values are presented relative to ITS. TYLCV-WT: wild-type TYLCV; TYLCV-C3mut: C3 null TYLCV mutant. Mean values are shown. Letters indicate a statistically significant difference according to one-way ANOVA-Welch (in b: degrees of freedom df = 3, F value = 50.98; in d: degrees of freedom df = 3, F value = 18.42; in e: degrees of freedom df = 3, F value = 17.70) followed by Games–Howell’s multiple comparison test (P < 0.05), or according to one-way ANOVA (in c: degrees of freedom df = 3, F value = 32.27) followed by Tukey’s multiple comparison test (P < 0.05). The original data from all experiments and replicates can be found in the Source data file.