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. 2021 May 13;12:2773. doi: 10.1038/s41467-021-22674-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Photographs and H&E staining images of the 4T1 tumour metastasis in the lung post-treatment with PBS and Vadimezan. Black arrows marked the haemorrhaging sites within the tumour, scale bar: 100 µm. b Fluorescence images showing the accumulation of aPDL1-Cy5.5 in the tumour metastases within the lung. c Quantitative analysis of the fluorescence intensity of aPDL1 in the lungs, n = 3 biologically independent animals, data are prestented as mean ± SD. d Confocal images showing the aPDL1 distribution within metastases in the lung. Metastatic tumour cells expressed GFP (green), blood vessels were marked by AF568-anti-CD31 antibody (red), and the aPDL1 was marked by Cy5-conjugated anti-rat IgG antibody (magenta), nuclei were labelled by Hoechst 33342 (blue), scale bar: 50 µm. Data are presented as mean ± SD, statistical significance was analysed via ANOVA (one-way, Tukey post-hoc test). P-value: ** P < 0.01.