Table 1.
Tandem mass spectrometry parameters and multiple reaction monitoring (MRM) transitions in positive and negative electrospray ionization mode.
| Compound | Retention time (min) | MRM transition (m/z) | Collision energy (eV) | Ionization mode |
|---|---|---|---|---|
| Chlorpropamide | 5.6 | 275.7 → 191.0, 127.1, 80.2 | 12, 22, 35 | Negative |
| γ-CEHC | 5.9 | 263.3 → 136.1, 149.1 | 23, 17 | Negative |
| α-CEHC | 6.2 | 277.3 → 233.1, 163.1, 150.1 | 10, 19, 15 | Negative |
| α-CMBHC | 7.5 | 319.4 → 150.1, 163.1, 275.1 | 15, 20, 14 | Negative |
| CUDA | 7.8 | 339.0 → 214.1, 240.1 | 22, 14 | Negative |
| γ-TT | 10.9 | 411.0 → 151.0, 191.0, 205.1 | 18, 13, 11 | Positive |
| α-TP | 12.6 | 431.7 → 165.0, 137.1, 111.2 | 18, 36, 14 | Positive |
| α-TT | 11.2 | 425.7 → 165.0, 205.1, 137.1 | 16, 12, 33 | Positive |
| γ-TP | 12.2 | 417.7 → 151.0, 123.1, 97.2 | 15, 37, 15 | Positive |
| α-TP-D6 | 12.6 | 437.4 → 171.1, 143.1, 97.2 | 18, 37, 17 | Positive |
Product ions used for quantitation are shown in bold.