Table 3.
Intra-batch accuracy and precision, and inter-batch accuracy and precision using liquid chromatography–mass spectrometry.
| Analyte | Nominal concentration (ng/mL) | Day 1 |
Day 2 |
Day 3 |
Inter-day |
||||
|---|---|---|---|---|---|---|---|---|---|
| Accuracy* (%) | Precision† (%) | Accuracy (%) | Precision (%) | Accuracy (%) | Precision (%) | Accuracy‡ (%) | Precision§ (%) | ||
| α-TP | 2.50 | 114 | 6.58 | 119 | 2.50 | 109 | 5.00 | 114 | 5.83 |
| 250 | 99.1 | 5.23 | 115 | 3.63 | 101 | 6.14 | 105 | 7.96 | |
| 800 | 103 | 4.95 | 108 | 2.23 | 102 | 4.44 | 104 | 4.55 | |
| γ-TP | 2.50 | 102 | 17.5 | 81.4 | 9.63 | 103 | 5.67 | 95.6 | 15.8 |
| 250 | 113 | 7.52 | 76.9 | 4.53 | 82.6 | 6.99 | 93.7 | 19.6 | |
| 800 | 101 | 8.57 | 79.3 | 21.5 | 84.0 | 7.80 | 88.2 | 16.5 | |
| α-TT | 2.50 | 84.3 | 5.10 | 80.4 | 9.97 | 92.6 | 7.95 | 85.8 | 9.53 |
| 250 | 94.6 | 7.98 | 109 | 4.39 | 109 | 7.12 | 104 | 9.11 | |
| 800 | 91.9 | 14.9 | 107 | 2.03 | 109 | 1.58 | 103 | 10.6 | |
| γ-TT | 2.50 | 115 | 8.06 | 93.1 | 18.7 | 106 | 5.21 | 104 | 13.7 |
| 250 | 75.7 | 6.63 | 76.5 | 22.1 | 73.9 | 11.5 | 75.3 | 14.0 | |
| 800 | 78.0 | 15.3 | 87.7 | 11.4 | 77.2 | 3.29 | 80.9 | 12.0 | |
| α-CMBHC | 2.50 | 99.5 | 7.11 | 116 | 2.86 | 98.8 | 24.4 | 105 | 8.88 |
| 250 | 98.3 | 5.60 | 116 | 0.96 | 98.9 | 11.8 | 105 | 10.6 | |
| 800 | 101 | 12.5 | 109 | 3.14 | 107 | 2.87 | 106 | 7.57 | |
| α-CEHC | 2.50 | 114 | 5.65 | 118 | 2.88 | 110 | 4.12 | 114 | 10.2 |
| 250 | 108 | 5.46 | 118 | 2.33 | 116 | 4.18 | 114 | 5.48 | |
| 800 | 101 | 11.5 | 110 | 1.96 | 112 | 2.81 | 108 | 7.46 | |
| γ-CEHC | 2.50 | 114 | 2.87 | 124 | 3.27 | 115 | 5.48 | 118 | 5.29 |
| 250 | 117 | 1.88 | 121 | 3.55 | 113 | 6.80 | 117 | 5.07 | |
| 800 | 109 | 6.02 | 108 | 2.47 | 118 | 6.09 | 111 | 6.20 | |
Intra-day accuracy was measured as the amount of target analytes added to a blank matrix, carried throughout the extraction procedure, and then analyzed. The % accuracy was based on the mean of 5 samples at low, medium, and high concentration levels.
Inter-day precision was measured as the amount of target analytes added to a blank matrix, carried throughout the extraction procedure, and then analyzed. The inter-day % precision is determined by the average % recoveries obtained in days 1–3.
Intra-day accuracy was measured by the amounts obtained from the coefficients of variation (% CV). The % accuracy is based on the averages of the % CVs of 5 samples at low, medium, and high concentration levels.
§ Inter-day precision was measured by the amounts obtained from the coefficients of variation (% CV). The inter-day precision % is determined by the average % precisions obtained in days 1–3.