Figure 3.

(a) RT-qPCR analysis of selected DERs and DUX4 target genes in myocytes differentiated from FSHD1 and FSHD2 patient-derived iPS cells. L1ME1 and MER72 were specifically upregulated in both FSHD1- and FSHD2-derived myocytes. The expression of MYH3 was measured as a muscle differentiation marker. Data are mean ± SEM. The differences are tested by one-way ANOVA followed by Tukey’s Multiple Comparison Test. P-values are as follows: ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001. (b) Expression level of DUX4 well correlated with the expression levels of L1ME1 and MER72. Pearson correlation coefficients are shown.