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. 2021 Apr 30;12(12):3648–3659. doi: 10.7150/jca.52851

Figure 3.

Figure 3

NRP1 overexpression promoted proliferation, migration and invasion in GC cells. (A) The expression of NRP1 in 5 GC cell lines (SGC-7901, MGC-803, BGC823, AGS, and MKN45) and 1 immortalized gastric mucosa cell, GES-1, were analyzed by western blotting (top panel) and qRT-PCR (bottom panel), respectively. (B) The expression of NRP1 in SGC-7901 cells transfected with vector or NRP1 overexpression plasmid (Left panel) and MGC-803 cells with NRP1-specific siRNA (Right panel) were detected by western blotting and qRT-PCR. (C) Transwell migration and matrigel invasion assays were conducted in SGC-7901 cells transfected with vector and NRP1 overexpression plasmid. Data were mean ± SD. (D) Transwell migration and matrigel invasion assays were conducted in MGC-803 cells with control siRNA (siCT) or two NRP1-specific siRNA (si#1/si#2). Data were mean ± SD. (E) Cell proliferation was evaluated by the CCK-8 assay. These data was obtained from three independent experiments. GC, gastric cancer; NRP1, Neuropilin-1; *, P< 0.05; **, P< 0.01; ***, P< 0.001; ns, no significant.