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. 2021 Apr 30;12(12):3648–3659. doi: 10.7150/jca.52851

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NRP1 promoted EMT in GC cells. (A-B) The expression of epithelial markers (E-cadherin), mesenchymal marker (Vimentin and N-cadherin), and transcriptional factors (Snail and Twist) were examined by western-blotting in SGC-7901 cells with NRP1-overexpressing plasmid. (C-D) The expression of epithelial markers (E-cadherin), mesenchymal marker (Vimentin and N-cadherin), and transcriptional factors (Snail and Twist) were examined by western-blotting in MGC-803 cells with NRP1-siRNA. (E) Immunofluorescence staining of SGC-7901 cells with vector or NRP1-overexpress plasmid, and MGC-803 cells with control or NRP1-siRNA. E-cadherin and Vimentin were red, the nuclei of the cells were blue. (F) NRP1-overexpressing in SGC-7901 cells induced EMT with morphological transformation and alterations in cellular configuration. Scale bars, 50μm (E), 200μm (F). These data were obtained from three independent experiments. GC, gastric cancer; NRP1, Neuropilin-1; *, P< 0.05; **, P< 0.01; ***, P< 0.001; ns, no significant.