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. 2021 May 3;12(12):3726–3740. doi: 10.7150/jca.48418

Figure 6.

Figure 6

Re-expression of miR-146b-3p regulates the expression of DYRK2 and GIL2. A) and B), Analysis of the gene expression levels of DYRK2 (A) and GIL2 (B) by real time PCR in 12 matched normal pancreatic tissue and pancreatic cancer tissues and (right) the data were analyzed using the Mann-Whitney U test. T, pancreatic cancer tumor tissue; N, pair-matched adjacent non-tumor tissue. C) Analysis of the protein expression levels of DYRK2 and GIL2 by western blotting in 12 matched normal pancreatic tissue and pancreatic cancer tissues and (D and E) the data were analyzed using the Mann-Whitney U test. T, pancreatic cancer tumor tissue; N, pair-matched adjacent non-tumor tissue. F and G) Correlation of the protein expression of DYRK2 and GIL2 with miR-146b-3p expression in 12 pairs of pancreatic cancer tissues and matched normal pancreatic tissues. Coefficient of determination from logarithmic regression model (R2) and P values are given. H) Analysis of the protein expression levels of DYRK2 and GIL2 in six pancreatic cancer cell lines by western blotting. I) Analysis of Gene expression of DYRK2 and GIL2 in Mia PaCa-2 and BxPC-3 cells after transfection with miR-146b-3p mimic or mimic NC or no transfection by real time PCR. J) Analysis of protein expression of DYRK2 and GIL2 in Mia PaCa-2 and BxPC-3 cells after transfection with miR-146b-3p mimic or mimic NC or no transfection by western blot. K) Working model for miR-146b-3p-MAP3K10-Gli2 axis.