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. 2021 Apr 24;12(12):3548–3557. doi: 10.7150/jca.50292

Figure 6.

Figure 6

Lenvatinib inhibits the expression of p-AKT and enhances GEM-mediated cell growth inhibition in GBC-SD and NOZ cells by inactivating the expression of p-AKT. GBC-SD and NOZ cells were pretreated with lenvatinib for 1 h, then treated with or without lenvatinib for 48 h. (A and B) The protein expression levels of p-AKT and PAPR1 were detected by western blotting with β-actin or GAPDH as a loading control. (C and D) Cell growth proliferation was detected by CCK-8. (E and F) The protein expression levels of p-AKT were measured by western blotting in GBC-SD and NOZ cells following treatment with lenvatinib and GEM. *P<0.05 vs. NC. **P<0.05 vs. GEM group. NC, negative control; GEM, gemcitabine; PAPR1, poly ADP-ribose polymerase 1; Len, lenvatinib.