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. 2021 Apr 19;12(12):3458–3467. doi: 10.7150/jca.56056

Figure 2.

Figure 2

Detection of IGHG1 expression level. (A) Western blotting was used to detect the expression of IGHG1 protein in different gastric cancer cell lines (BGC823, MGC803, SGC7901) and immortalized gastric mucosal cells GES-1. IGHG1 was highly expressed in gastric cancer cell lines, and the highest expression was found in SGC7901 cells. (B) Fluorescent expression efficiency of gastric cancer cell SGC7901 after transient transfection of pPLK-shRNA-IGHG1. Original magnification, 100×. Scale bar: 200 μm. (C) Western blotting was used to detect the expression of IGHG1 protein after transfection of pPLK-shRNA-IGHG1 (shRNA-NC, shRNAa, shRNAb, shRNAc), and the expression level of IGHG1 protein was the lowest in shRNAc group. *Compared with the control group, P<0.05.