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. 2021 Apr 15;11(13):6225–6239. doi: 10.7150/thno.55939

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DHA exhibits anti-tumor effects in colon cancer cells. (A) Viabilities of HCT116 and RKO cells treated with various concentrations of DHA at different times. (B) Expression levels of apoptosis-associated proteins in HCT116 and RKO cell lines treated with DHA. (C) Flow cytometric analysis of apoptosis in HCT116 and RKO cell lines treated with DHA and Z-VAD-FMK. (D) The percentage of apoptotic cells was measured. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. All experiments were repeated three times.