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. 2021 May 3;11(13):6592–6606. doi: 10.7150/thno.59816

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MYOCD recruits PRMT5/MEP50 methyltransferase complex to TGFBR2 promoter region. (A) Silver-staining of SDS-PAGE separated protein samples immunoprecipitated with antibody against Flag from 293T-GFP 3×FLAG and 293T-MYOCD 3×FLAG cells. (B-C) MYOCD is associated with PRMT5 and WDR77. The 293T cells were transfected with the indicated plasmids (2.5 μg each). Coimmunoprecipitation and immunoblot were performed with the indicated antibodies. (D) Endogenous MYOCD is associated with PRMT5 and WDR77 in A549 cells. Coimmunoprecipitation experiments were performed with indicated antibodies, and the immunoprecipitates and WCL were analyzed with the indicated antibodies. (E-F) MYOCD, PRMT5, WDR77 and H3R8mes localized in TGFBR2 promoter region. (E) ChIP-PCR analysis of the TGFBR2 promoters was performed using indicated antibodies in A549 cells; (F) Re-ChIP analysis of the TGFBR2 promoters was performed using antibodies against indicated antibodies in A549 cells.