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. 2021 Apr 27;11(13):6560–6572. doi: 10.7150/thno.55609

Figure 3.

Figure 3

Cholesterol biosynthesis pathway is required for CRC cell growth. A-C, Confirmation of SREBP2 knockdown in HT29 cells and SW620 cells determined by western blotting (A) and quantitative RT-PCR (B and C). D, E, The effect of SREBP2 knockdown on cell proliferation in HT29 cells (D) and SW620 cells (E). The relative cell number was expressed as fold change to Day 0. F-H, The effect of SREBP2 knockdown on colony formation in HT29 cells and SW620 cells. Briefly, 500 cells were plated in triplicate on a six-well plate and cultured for two weeks. The representative photo is shown in (F). I, J, The effect of SREBP2 knockdown on total cholesterol levels in HT29 cells (I) and SW620 cells (J). Representative results from at least three independent experiments are shown. K, L, cholesterol supplementation reverses the effect of SREBP2 knockdown on cell proliferation in HT29 cells (K) and SW620 cells (L). SREBP2 knockdown HT29 cells were treated at 0, 0.5 or 1 µg/mL for 24 h. Data are shown as mean ± SD of triplicate or quadruple experiments (B-E and G-L). Significance was determined by two-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001.