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. 2021 Apr 30;11(13):6616–6631. doi: 10.7150/thno.57701

Figure 5.

Figure 5

PEP biogel activated TGF-β signaling and promoted collagen organization. (A): Masson Trichrome staining analysis of different groups of wound tissue, as well as normal rabbit ear skin. Skin tissue was obtained at day 28 post-surgery. (B): TGF-β immunofluorescence staining of wounds from different groups. (C): Col1A and Col3A immunofluorescence staining of wounds from different groups. (D), (E): Immunofluorescence quantification for TGF-β, COL1A, COL3A and COL3A to COL1A ratio, N=8. (F): Cyclic tensile test for all the groups. Untreated and TISSEL treated only groups were stiffer and less like normal skin. Rc: reaction force variation. N=4. (G): Maximum tensile test of all the groups. The TISSEEL-PEP group resisted the highest tensile forces, N=4. Scale bar in Figure A: 100 µm. Scale bar in B&C: 200 µm. 2-way ANOVA for each group compared with control group in Figures D and E. 2-tailed unpaired Student's t-test for each group compared with untreated control group in Figure B,C&G. *p < 0.05, **p < 0.01, ***p < 0.001.