Figure 3.

Ability of RGD-EV_ReN to target the lesion region of ischemic brain. (A) TTC-stained brain sections show the typical infarct area in a mouse receiving 1 h of MCAO and 24 h of reperfusion. (B) Representative NIRF images (overlaid with photograph) of mice brains which received MCAO/R and the administration of PBS, Cy5.5-labeled EV_ReN, Scr-EV_ReN or RGD-EV_ReN. Brains were dissected 24 h after administration (36 h after reperfusion). (C) Quantitation of fluorescence intensity (n = 5) in the lesion region. (D) Ratios of fluorescence intensity (n = 5) in ipsilateral versus contralateral region. Data in C and D are expressed as mean ± SEM. **P < 0.01 by One-way ANOVA. (E) Representative NIRF images of organs dissected from mice which received MCAO/R along with the administration of PBS, Cy5.5-labeled EV_ReN or RGD-EV_ReN. Organs were dissected 24 h after administration (36 h after reperfusion). Right boxed graph illustrates location of the analyzed six organs. (F) Quantitation of fluorescence intensities (n = 6) per gram of tissue in different organs. Data are expressed as mean ± SEM. *P < 0.05, ***P < 0.001 by One-way ANOVA.