Figure 4.

Recognition of integrin β₃ on reactive endothelial cells by RGD-EV_ReN. (A) Co-labeled fluorescence images of vascular endothelial cells (CD34, green) with integrin β₃ (red) in the lesion region (ipsilateral) and non-ischemic region (contralateral) 12 h after reperfusion. (B) Schematic diagram of RGD-EV_ReN binding integrin α_vβ₃ on reactive endothelial cells in ischemic brain after intravenous administration. (C,D) Co-labeled fluorescence images of integrin β₃ or CD34 (green) with EV_ReN, Scr-EV_ReN, or RGD-EV_ReN (red) in the lesion region 6 h after intravenous administration (18 h after reperfusion). Blue indicates nuclei. Scale bars, 50 µm.