Figure 2.
Activity of HDA15 promoter in response to salt stress. The promoter of HDA15 was cloned into a pMDC162 vector containing the GUS reporter gene via the Gateway system. Homozygous T4 seeds were used to perform β-glucuronidase (GUS) staining and quantification. (A) Visualization of the response of the HDA15 promoter to salt stress. Seven-day-old plants were stained with GUS solution for 2 h to visualize promoter activity in response to 150 mM NaCl at 6 and 24 h. (B) GUS quantification of the HDA15 promoter under salt stress. RNA was extracted from 7-day-old HDA15pro::GUS plants challenged by salt stress under the same conditions indicated above for GUS staining. The GUS primer was used to perform qRT-PCR. Actin2 was used as an internal control. Error bars represent the standard deviation of three replicates. Different letters (a, b) within a treatment group indicate significant differences based on one-way ANOVA (P < 0.05).