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. 2021 Apr 30;12:640443. doi: 10.3389/fpls.2021.640443

Figure 9.

Figure 9

The co-action of HDA15 and HY5 in response to salt stress. Three-day-old plants germinated in normal MS media were transferred to different NaCl concentrations as indicated. The experiment was repeated three times independently to obtain a consistent result. (A) The phenotype of Col-0, HDA15 OE/hy5 ko, hy5 ko mutants, and HDA15 OE plants following a 4 d exposure to salt stress. (B) Chlorophyll levels of tested plants following a 4 d exposure to salt stress. (C) The expression levels of HY5 in Col-0 and HDA15 OE plants. (D,E) The expression levels of NCED3 and RD29B in Col-0, double mutants, HDA15 OE, and hy5 ko plants. Seven-day-old plants were exposed to 150 mM NaCl for 6 h followed by RNA extraction and cDNA synthesis for qRT-PCR. Actin2 was used as an internal control. Error bars represent the standard deviation of three replicates. Different letters (a, b, or c) within a treatment group indicate significant differences based on one-way ANOVA (P < 0.05).