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. 2021 Apr 30;9:634649. doi: 10.3389/fcell.2021.634649

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Copyright © 2021 Conduit, Davies, Fulcher, Oorschot and Mitchell.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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INPP5E exhibits a ring-shaped localization at the transition zone. (A) hTERT-RPE1 cells were transfected with GFP-INPP5E, serum starved and immunostained with GFP (green) and ARL13B (grayscale) antibodies and imaged by STED microscopy (confocal resolution image of the ARL13B stained axoneme is shown). Arrows indicate transition zone INPP5E localization, bar indicates 1 μm. (B) Graph shows the lateral diameter between the highest intensity points of the INPP5E transition zone protein puncta perpendicular to the plane of the axoneme. Bars represent mean ± SEM, n = 3 independent experiments, 30 cilia imaged per experiment and all cilia with two distinct INPP5E puncta measured. (C) Example image showing method for the lateral diameter and axial distance measurements, bar indicates 1 μm.