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. 2021 Apr 30;9:667435. doi: 10.3389/fcell.2021.667435

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Copyright © 2021 Du, Peng, Gai, Liu, Liu, Chen, Li, Zhang, Liu, Zhang, Jiang and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PTEN is indispensable for MLN4924 to inhibit PI3K/Akt signaling pathway activity. (A) Immunoblot of WCL from MCF-7 cells and BT-549 (PTEN-null) cells with MLN4924 treatment (1 μM) for 12 h. (B) Immunoblot of WCL from shNC/Control or shPTEN/sgPTEN MCF-7 cells. The cells were treated with 1 μM MLN4924 for 12 h. (C,D) Quantitative mRNA level analysis of p27^kip1 and p15^INK4b in shNC and shPTEN MCF-7 cells. MLN4924 was used at indicated dose (1 μM; 12 h). The quantifications of indicated protein contents were analyzed. *P < 0.05, **P < 0.01, ***P < 0.001.