TABLE 1.

Monoclonal antibody pair matching test for sandwich ELISA (OD at A450 nm)

mAb	Bio‐P1	Bio‐P2
A0‐A7‐G11	1.66	1.51
B6‐A1‐B11	1.17	1.64
C7‐C4	0.72	1.10
A2‐D8	0.04	0.26
5‐D10	0.19	0.35

Note: Coated with 100 μl of 3 μg/ml mAb, rPru p 1 1 μg/ml (rPru p 1.0101 and rPru p 1.0201, 1:1 mixed), detector pAb 100 μl of 3 μg/ml.

Abbreviations: ELISA, enzyme‐linked immunosorbent assay; OD, optical density.