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The percent recovery and the coefficients of variation of sandwich ELISA
| Spiked rPru p 1 (ng/ml) | Intra‐assay (n = 6) | Inter‐assay (n = 3) | |||||
|---|---|---|---|---|---|---|---|
| Measured (ng/ml) | SD (ng/ml) | CV (%) | Recovery rate | Measured (ng/ml) | SD (ng/ml) | CV (%) | |
| 4 | 4.54 | 0.024 | 6.74 | 113.5 | 4.1 | 0.03 | 7.58 |
| 8 | 8.09 | 0.021 | 4.02 | 101.1 | 7.71 | 0.02 | 4.96 |
| 16 | 16.05 | 0.024 | 2.88 | 100.3 | 15.81 | 0.02 | 1.96 |
| 32 | 32.53 | 0.091 | 7.25 | 101.6 | 29.80 | 0.04 | 3.04 |
Abbreviation: ELISA, enzyme‐linked immunosorbent assay.
