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. 2021 May 3;22(1):711. doi: 10.3892/etm.2021.10143

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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STAT3 knockdown promotes neuronal differentiation in NSCs. (A) Immunofluorescence indicated that the NSC-specific marker nestin was highly expressed in cells. (B and C) Western blotting was performed to measure STAT3 protein levels in each group. (D) Effect of STAT3 gene silencing on STAT3 mRNA expression measured using reverse transcription-quantitative PCR. (E) Immunostaining for β3-tubulin and GFAP (red), GFP (green) in each group. Quantification of the percentage of (F) β3-tubulin- and (G) GFAP-positive cells in each group. Scale bar, 50 µm. ^*P<0.05 and ^**P<0.01 vs. LV-CL. NSCs, neural stem cells; GFP, Green fluorescent protein; GFAP, fibrillary acidic protein; LV, lentivirus; CL, control.