FIGURE 3:

Cell-specific pHlys. (A) Average steady-state pHlys in RPE cells stably expressing pHLARE (n = 74 cells) and in MCF10A (23 cells), MDCK (n = 17 cells), NIH-3T3 (15 cells), and CCL39 (n = 11 cells) transiently expressing pHLARE and maintained in growth medium. Box plots show median, first, and third quartile, with whiskers extending to observations within 1.5 times the interquartile range, and all individual data points include data for individual cells obtained from three to seven separate cell preparations. Statistical analysis by Tukey–Kramer HSD test. (B) Steady-state pHlys of lysosomes within 3 μm of the nuclear membrane (perinuclear) and 3 μm of the distal margin of membrane protrusions in RPE cells, indicated by hatched lines. Data are expressed as described in A and obtained from six separate cell preparations. (C–E) Lysosome motility, including mean speed (C), maximum speed (D), and displacement (E) of RPE control cells and RPE-pHLARE cells. Plots indicate mean and SEM with Student’s t test from three separate cell preparations.