FIGURE 6:

Hyperactive Vps34-EDC may lead to a modest decrease in homotypic vacuole fusion. (A–C) Hyperactive Vps34-EDC results in a 12% increase in the average number of vacuole lobes in a cell (B), which occurs primarily due to a decrease in the number of cells with 1 vacuole lobe (C). vps34Δ cells were transformed with pRS416-Vps34 or pRS416-Vps34-EDC. After labeling with FM 4-64, cells were chased at 24°C for 3 h before imaging. DIC, differential interference contrast. Scale bar = 5 µm. The number of vacuole lobes was counted by a scorer who was blinded to the genotype of the cells being quantified. Quantification of at least 100 cells per n, n = 4. Error bars indicate SD. Unpaired t test (B). Two-way ANOVA and Dunnett’s post-hoc test (C). ns = p > 0.05, * = p < 0.05, ** = p < 0.01.