Fig. 3.

The PTT effects was evaluated in vitro and in vivo. a 4T1 cells viability after incubation with HPP with or without 1064 nm laser. b Live and dead staining of 4T1 cells treated under different conditions (Scale bar = 200 μm). c In vivo IR thermal images of 4T1 tumor-bearing mice intratumorally injected with PBS and HPP dispersion activated by 0.6 W/cm² 1064 nm laser for 7 min. d Temperature increased profile of tumor area under laser irradiation. *p < 0.01