Figure 3.

Maximum likelihood phylogenetic tree based on the Usutu virus non-structural protein 5 gene sequences, showing the relatedness of strains infecting mosquitoes in Slovakia to Usutu virus lineage Europe 2, 2018, 2019 (n = 34 sequences in tree)

The tree was generated the using Kimura-2 model with bootstrap resampling of 1,000 replicates. The tree is drawn to scale, with branch lengths corresponding to the number of substitutions per site. Bootstrap values are shown at the nodes. GenBank accession numbers of the sequences in the tree are provided and the sequences retrieved in the current study are in bold font and further indicated by black circles.