Figure EV2. Light‐induced dissipation of ERK phosphorylation is signaling‐dependent. Related to Fig 3 .

1. OptoCAR^C450G‐T cells were conjugated as for the main experiments shown in Fig 3 but exposed to continuous illumination from dimerizer addition at 0 min for 15 min. Left panel shows the median ppERK intensity. Bars show mean ± SEM (n = 3). Right panel shows representative ppERK distributions from this control experiment.
2. OptoCAR^I539E‐T cells were conjugated as for the main experiments shown in Fig 3 and remained in the dark state from dimerizer addition at 0 min for 15 min. Left panel shows the median ppERK intensity. Bars show mean ± SEM (n = 3). Right panel shows representative ppERK distributions from this control experiment.