Fig. 2.

Knockdown of METTL13 promotes ccRCC cell proliferation, viability, migration and invasion. a METTL13 protein expressions in five ccRCC cell lines and one immortalized ccRCC cell line by western blotting. b Knockdown efficiencies of two siRNAs targeting METTL13 in two cell lines detected by western blotting. c-e Cell proliferation, viability and migration were measured by EdU assay (c), CCK-8 assay (d) and wound-healing assay (e), respectively. f Cell migration assay and invasion assay were respectively used to detect cells’ migratory ability and invasiveness. g Expression levels of EMT-related proteins (N-cadherin, E-cadherin) from cells were detected via western blotting. Each experiment was conducted independently at least three times. Student’s t-test (two-tailed) was used for statistical analysis, *P < 0.05, **P < 0.01, ***P < 0.001. Bar graphs: mean ± S.D., n = 3