Fig. 6.

METTL13 negatively regulates PI3K/AKT/mTOR/HIF-1α signaling pathway. a, b Protein levels (a) and mRNA levels (b) of HIF-1α were respectively detected by western blot and qRT-PCR after silencing or overexpressing METTL13. c, d Protein levels of p-PI3K, PI3K, p-AKT, AKT, p-mTOR and mTOR were determined by western blot after knocking down METTL13 (c) or overexpressing METTL13 (d). Each experiment was carried out at least for three times independently. Student’s t-test (two-tailed) was used for statistical analysis, *P < 0.05, **P < 0.01, ***P < 0.001. Bar graphs: mean ± S.D., n = 3