Fig. 8.

METTL13 inhibits ccRCC cells’ growth and metastasis in vivo. a Image of BALB/c nude mice executed 30 days after injection of cells transfected with empty vector or with METTL13 overexpression plasmid. b Image of excised tumors from the mice. c The weights of the isolated tumors. d western blot was used to measure protein expressions of METTL13, HIF-1α and c-Myc in the nude mice tumors. e Cells overexpressing empty vector or METTL13 were injected into nude mice through tail vein. Representative images of lungs with metastatic tumors excised from the mice and H&E staining of the lung tissues were shown. f The numbers of metastatic tumors in lungs per field were measured under a magnification of 40x. Each experiment was conducted at least three times. Student’s t-test (two-tailed) was used for statistical analysis, *P < 0.05. Bar graphs: mean ± S.D., n = 3