Table 1.
Strains used in this study
| Name | Genotype | Source or reference |
|---|---|---|
| S. coelicolor | ||
| M145 (WT) | SCP1-SCP2- | [3] |
| PS04 (TopA↓) | M145 ΔtopA::scar attB ΦC31::pIJ6902topA | [62] |
| MG03 (WT-lux) | M145 attBΦBT1::pFLUXHptopA | [64] |
| MG04 (TopA↓-lux) | M145 ΔtopA::scar attBΦC31::pIJ6902topA attBΦBT1::pFLUXHptopA | [64] |
| MG02 | M145 attBΦBT1::pFLUXH_permE | [64] |
| MG01 | M145 attBΦBT1::pFLUXH | [64] |
| lux-tn66 | MG01 tn5::sco4804 | This study |
| lux-tn66RP | lux-tn66 pWHM3Spec | This study |
| MS10 | M145 pWHM3Hyg | [64] |
| MS11 |
M145 ΔtopA::scar attBΦC31::pIJ6902topA pWHM3Hyg |
[62] |
| MG66 | MG01 attBΦC31::pIJ6902_sco4804 | This study |
| MG66_RP | MG66 pWHM3Hyg | This study |
| M145_pIJ6902 (WTØ) | M145 attBΦC31::pIJ6902 | This study |
| MG03_pIJ6902 (WTØ-lux) | MG03 attBΦC31::pIJ6902 | This study |
| E. coli | ||
| DH5α | F–endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK–mK +), λ– | Lab stock |
| ET12567 pUZ8002 | dam-13::Tn9 dcm cat tet hsd zjj-201::Tn10 tra neo RP4 | [34] |
| BW25113/pIJ790 | K12 derivative; ΔaraBAD ΔrhaBAD λ-Red (gam bet exo) cat araC rep101(Ts) | Gust et al. [26] |
| BL21 (DE3) groEL-groES strain | F–ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB +]K-12(λS) pGroESL | [24] |